Phytochemical Characterization of Jujube (Ziziphus jujuba Mill.) Fruit
Keywords:
Ziziphus jujuba Mill., phenolic compounds, flavonoids, organic acids, antioxidant capacityAbstract
This study was conducted to determine the total phenolic content (TPC), total flavonoid
content (TFC), antioxidant activity (DPPH), and organic acid profiles of jujube (Ziziphus jujuba Mill.)
fruit extracts. The TPC, TFC, and DPPH values were found to be 379.33 mg GAE L⁻¹, 374.33 µg QE
mL⁻¹, and 89.67%, respectively. The low variation (SD ≤ 2.08) indicates high repeatability of the
measurements. Among the phenolic compounds, ellagic acid (9.00 mg 100 g⁻¹) and gallic acid (7.67 mg
100 g⁻¹) were detected at the highest levels, followed by protocatechuic acid (5.67 mg 100 g⁻¹) and
quercetin (2.33 mg 100 g⁻¹). Regarding organic acids, acetic acid (182.00 mg 100 g⁻¹), oxalic acid
(265.67 mg 100 g⁻¹), and malic acid (66.33 mg 100 g⁻¹) were identified as the predominant compounds.
An examination of their distribution in total fruit juice revealed that organic acids constituted the
majority, whereas phenolic compounds represented a smaller proportion. These findings demonstrate that
jujube fruit extracts possess a high antioxidant potential and that the fruit is rich in phytochemical
constituents, suggesting its potential use as a health-promoting functional food.
Downloads
References
Li, D., Yue, D., Liu, D., Zhang, L., & Song, S. (2020). Phytochemical and chemotaxonomic study on Ziziphus jujuba Mill. (Rhamnaceae). Biochemical Systematics and Ecology, 91, 104058. https://doi.org/10.1016/j.bse.2020.104058.
Zhu, D., Zhu, Y., Tan, H., Ding, R., Dai, Q., Du, X., Liu, Y., & Yue, R. (2024). Effects of jujube (Ziziphus jujuba Mill.) fruit extracts on oxidative stress: A systematic review and meta-analysis of rodent studies. Food Science & Nutrition, Advance online publication. https://doi.org/10.1002/fsn3.4234
Yahia, Y., Benabderrahim, M. A., Tlili, N., Bagues, M., & Nagaz, K. (2020). Bioactive compounds, antioxidant and antimicrobial activities of extracts from different plant parts of two Ziziphus Mill. species. PLoS ONE, 15(5), e0232599. https://doi.org/10.1371/journal.pone.0232599
Cai, W., Zhuang, H., Wang, X., Fu, X., Chen, S., Yao, L., Sun, M., Wang, H., Yu, C., & Feng, T. (2024). Functional Nutrients and Jujube-Based Processed Products in Ziziphus jujuba. Molecules, 29(14), 3437. https://doi.org/10.3390/molecules29143437.
Ishara, J., Buzera, A., Mushagalusa, G. N., Hammam, A. R. A., Munga, J., Karanja, P., & Kinyuru, J. (2021). Nutraceutical potential of mushroom bioactive metabolites and their food functionality. Journal of Food Biochemistry, 45(12), e14025. https://doi.org/10.1111/jfbc.14025.
Lin, Y.-S., Lin, W.-S., Tung, J.-W., Cheng, Y.-C., Chang, M.-Y., Chen, C.-Y., & Huang, S.-L. (2020). Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp. Applied Sciences, 10(17), 6007. https://doi.org/10.3390/app10176007.
Wen, C., Wang, C., Sun, P., Qiu, L., Wang, H., Qiu, Z., Wen, S., & Li, X. (2025). Metabolic characteristics and nutritional quality of fresh jujube (Ziziphus jujuba mill.) fruits in eleven producing areas. Food chemistry: X, 33, 103431. https://doi.org/10.1016/j.fochx.2025.103431.
Yakubu, H. B., Bichi, A. H., & Dauda, A. B. (2025). Influence of processing methods on the phytochemical, mineral and fibre fraction of jujube fruit meal as a potential fish feed ingredient. FUDMA Journal of Sciences, 9, 327–331. https://doi.org/10.33003/fjs-2025-0904-3602.
Gao, Q. H., Wu, C. S., & Wang, M. (2013). The jujube (Ziziphus jujuba Mill.) fruit: A review of current knowledge of fruit composition and health benefits. Journal of Agricultural and Food Chemistry, 61(14), 3351–3363. https://doi.org/10.1021/jf4007032.
Shi, Q., Zhang, Z., Su, J., Zhou, J., & Li, X. (2018). Comparative analysis of pigments, phenolics, and antioxidant activity of Chinese jujube (Ziziphus jujuba Mill.) during fruit development. Molecules, 23(8), 1917. https://doi.org/10.3390/molecules23081917.
Gamez-Meza, N., Noriega-Rodriguez, J. A., Medina-Juarez, L. A., Ortega-Garcia, J., Cazarez-Casanova, R., & Angulo-Guerrero, O. (1999). Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. JAOCS, Journal of the American Oil Chemists’ Society, 76(12), 1445–1447. https://doi.org/10.1007/S11746-999-0182-4.
Woisky, R. G., & Salatino, A. (1998). Analysis of propolis: Some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37(2), 99–105. https://doi.org/10.1080/00218839.1998.11100961.
Wen, D., Li, C., Di, H., Liao, Y., & Liu, H. (2005). A universal HPLC method for the determination of phenolic acids in compound herbal medicines. Journal of Agricultural and Food Chemistry, 53(17), 6624–6629. https://doi.org/10.1021/jf0511291.
Fu, H. X., Zhang, L. L., He, B., Yue, P. X., & Gao, X. L. (2015). Analysis of organic acids in blueberry juice and its fermented wine by high-performance liquid chromatography. Advance Journal of Food Science and Technology, 9, 127–134. https://doi.org/10.19026/ajfst.9.1947.
Ebrahimzadeh, M. A., Hosseinimehr, S. J., Hamidinia, A., & Jafari, M. (2008). Antioxidant and free radical scavenging activity of Feijoa sellowiana fruits peel and leaves. Pharmacologyonline, 1, 7–14.
Kamiloglu, O., Ercisli, S., Engül, M., Toplu, C., & Serce, S. (2009). Total phenolics and antioxidant activity of jujube (Zizyphus jujube Mill.) genotypes selected from Turkey. African Journal of Biotechnology, 8(2), 303–307.
Singh, B., Gautam, S., Dubey, B. K., & Basedia, D. K. (2022). Anti-inflammatory and antioxidant activity of Ziziphus jujuba extract. Asian Journal of Pharmaceutical Research and Development, 10(4), 115–121. https://doi.org/10.22270/ajprd.v10i4.1164.
Chiti, S., Basiri, S., Mortazavi, A., & Sharifi, A. (2019). Evaluation on physicochemical properties and antioxidant capacity of two Iranian jujube (Ziziphus jujuba Mill.) cultivars. Journal of Medicinal Plants and By-Products, 1(1), 85–93.
Bao, T., Zhang, M., Zhou, Y., & Chen, W. (2021). Phenolic profile of jujube fruit subjected to gut microbiota fermentation and its antioxidant potential against ethyl carbamate-induced oxidative damage. Journal of Zhejiang University. Science. B, 22(5), 397–409. https://doi.org/10.1631/jzus.B2000754.
Xue, X., Zhao, A., Wang, Y., Ren, H., Su, W., Li, Y., Shi, M., Liu, L., & Li, D. (2023). Metabolomics and transcriptomics analyses for characterizing the alkaloid metabolism of Chinese jujube and sour jujube fruits. Frontiers in Plant Science, 14, Article 1267758. https://doi.org/10.3389/fpls.2023.1267758.
Song, J., Bi, J., Chen, Q., Wu, X., Lyu, Y., & Meng, X. (2019). Assessment of sugar content, fatty acids, free amino acids, and volatile profiles in jujube fruits at different ripening stages. Food Chemistry, 270, 344–352. https://doi.org/10.1016/j.foodchem.2018.07.102.
Adjdir, S., Benariba, N., Laoufi, H., & Djaziri, R. (2019). Phenolic content and antioxidant activity of Ziziphus jujuba Mill. fruit extracts. Phytothérapie, 17(2), 74–82.
Xie, P. J., You, F., Huang, L. X., & Zhang, C. H. (2017). Comprehensive assessment of phenolic compounds and antioxidant performance in the developmental process of jujube (Ziziphus jujuba Mill.). Journal of Functional Foods, 36, 233–242. https://doi.org/10.1016/j.jff.2017.07.012.
Reche, J., Almansa, M. S., Hernández, F., Amorós, A., & Legua, P. (2021). Physicochemical and Antioxidant Capacity of Jujube (Ziziphus jujuba Mill.) at Different Maturation Stages. Agronomy, 11(1), 132. https://doi.org/10.3390/agronomy11010132.
Cai, W., Zhuang, H., Wang, X., Fu, X., Chen, S., Yao, L., Sun, M., Wang, H., Yu, C., & Feng, T. (2024). Functional Nutrients and Jujube-Based Processed Products in Ziziphus jujuba. Molecules, 29(14), 3437. https://doi.org/10.3390/molecules29143437
Zhu, D., Jiang, N., Wang, N., Zhao, Y., & Liu, X. (2024). A Literature Review of the Pharmacological Effects of Jujube. Foods, 13(2), 193. https://doi.org/10.3390/foods13020193.
Chen, J., & Tsim, K. W. K. (2020). A review of edible jujube, the Ziziphus jujuba fruit: A health food supplement for anemia prevalence. Frontiers in Pharmacology, 11, Article 593655. https://doi.org/10.3389/fphar.2020.593655.
WebMD. (2024). What to know about eating jujube fruit (Chinese dates). https://www.verywellhealth.com/jujube-8734898.
Hong, S., Kim, Y., Sung, J., Lee, H., Heo, H., Jeong, H. S., & Lee, J. (2020). Jujube (Ziziphus jujuba Mill.) protects hepatocytes against alcohol-induced damage through Nrf2 activation. Evidence-Based Complementary and Alternative Medicine, Article 6684331. https://doi.org/10.1155/2020/6684331.