Production and purification of laccase from Trametes versicolor


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Authors

  • Semih Işık Biology, Graduate School of Natural and Applied Sciences, Kocaeli University, Turkey
  • Simge Çolak Biology, Graduate School of Natural and Applied Sciences, Kocaeli University, Turkey
  • Yonca Yüzügüllü Karakuş *yonca.yuzugullu@kocaeli.edu.tr

Keywords:

Trametes Versicolor, Laccase,, Medium, Purification, Three-Phase Partitioning

Abstract

This study describes the production and purification of laccase from Trametes versicolor isolate. The activated fungus was grown in 50 mL YpSs broth medium for 3 days at 30 °C and 120 rpm shaking speed. Then, pellets were transferred to the laccase production medium including 5 g/L glucose, 1 g/L yeast extract, 1 g/L beef extract, 0.2 g/L pepton, 1 g/L KH2PO4, 0.5 g/L MgSO4, 0.1 g/L CuSO4, 3 g/L (NH4)2SO4, 1 g/L CaCl2, and 1 g/L Na2HPO4. The highest enzyme activity was found after the sixth day of cell growth. For purification of laccase from crude extract, three-phase partitioning was used for the first time. The enzyme was purified by 9.5-fold with an enzyme activity recovery of 93% from the TPP system prepared at pH 7.0 with a ratio of 1:1 crude extract:t-butanol and containing 30% (w/w) ammonium sulphate. As a result, it is shown that the laccase enzyme from T. versicolor can be purified using three-phase systems, which are timesaving, inexpensive, and easy to use, instead of the traditional chromatography method.

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Published

2023-02-08

How to Cite

Işık, S., Çolak, S., & Yüzügüllü Karakuş, Y. (2023). Production and purification of laccase from Trametes versicolor. International Conference on Frontiers in Academic Research, 1, 551–555. Retrieved from https://as-proceeding.com/index.php/icfar/article/view/162