Detection of Tp73 Gene Polymorphism, Sequencing and Gene Expression in Women Suffered From Breast Cancer in Ninewa - Mosul, Iraq.

Abstract

Two novel members of the P53 family were discovered in 1997 and given the names p73, and p63. p73, have numerous isoforms. Based on the resultant structure, two classifications can be created for all p73 isoforms, (TAp73), which activate transcription that exhibits tumor-suppressor activity. (DNp73) that act as dominant-negative repressors and operate as proto-oncogenes. It was identifying new variations of the TP73 gene, which may be directly or indirectly affecting the activity of this gene, which is the cause of Among the main reasons for the development of breast tumour diseases, as it appeared in the results of sequencing tests to amplify the TP73 gene, the presence of previously unrecorded or unregistered differences in the sequences of a number of nucleotides. The results of the reaction of the Tetra ARMS PCR showed that there is a relationship between patients suffering from breast tumours and the genetic variation of the TP73 gene at the site (TP73G4C14- A4T14), as it was clear from the results of the PCR reaction that the genetic variation appeared The TP73 gene was found in three different genotypes, in different proportions. The results of the response of the Tetra ARMS PCR reaction showed, that there is a relationship between patients suffering from breast tumours and the genetic variation of the TP73 gene at the site (rs1801173), as it was clear from the results of the PCR reaction that the genetic variation of the gene TP73 in three different genotypes, in different proportions.