Exploration of the biodiversity of the lactic flora of a traditional Algerian cheese (type: Jben) from the steppe zones of the Algerian center of Djelfa.


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Authors

  • Ismail BENZEKRI University of Mostaganem
  • Abderrahim CHERIGUENE University of Mostaganem
  • Fadéla CHOUGRANI University of Mostaganem
  • Abdelkader Elamine DAHOU University of Mostaganem

Keywords:

Exploration, Biodiversity, Lactic Flora, Jben, Steppe Zones

Abstract

Five samples of traditional Algerian cheese (type: Jben) collected from five different sites in
the Algerian center of Djelfa's steppe zones, were studied by determining its physicochemical and
microbiological characteristics. The diversity and density of the lactic acid bacteria of this cheese have
been the subject of phenotypic, physiological, and biochemical analyses. The average pH of the samples
was 4.39 ± 0.15, the average titratable acidity value was 69.4 ± 3.11 D and the results of the
microbiological analyses were; total aerobic mesophilic flora 6.34 x 106 CFU/g, coliform bacteria 1.44 x
104 CFU/g, Yeasts 1.88 x 104 CFU/g, Staphylococcus, Salmonella, and Molds were not detected. Among
the lactic microflora studied, 164 strains of lactic acid bacteria were isolated and purified. This study
revealed the presence of 101 Cocci-shaped and 63 rod-shaped. The Coccids are represented by the
Enterococcus, Lactococcus, Leuconostoc, and Pediococcus; the genus Lactobacillus is detected and
identified in varying proportions. This diversity could be developed to have an interest in the cheese
manufacturing of the Jben type, particularly in its valorization, and thus could be used as a probiotic
indication for health.

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Author Biographies

Ismail BENZEKRI , University of Mostaganem

Department of Food Science, Laboratory of bioeconomy, Food Security and Health, Algeria.

Abderrahim CHERIGUENE , University of Mostaganem

Department of Food Science, Laboratory of bioeconomy, Food Security and Health, Algeria.

Fadéla CHOUGRANI , University of Mostaganem

Department of Food Science, Laboratory of Sciences and Techniques of Animal Production, Algeria.

Abdelkader Elamine DAHOU , University of Mostaganem

Department of Food Science, Laboratory of Sciences and Techniques of Animal Production, Algeria.

References

S. Hadef, Evaluation of the technological and probiotic abilities of local lactic acid bacteria. Master's thesis, Kasdi Merbah University of Ouargla. Applied microbiology option, 2012, 87p.

I. Verdier-Metz, V. Michel, C. Belbes and M. Montel, Do milking practices influence the bacterial diversity of raw milk? Food Microbiology 26, 2009,305-310.

A. Mechai, D. Kirane, Antimicrobial activity of autochthonous lactic acid bacteria isolated from Algerian traditional fermented milk “Raib”. African. J. Biotechnology: vol 7 N° 15, 2008, 2908-2914.

J.P. Larpent, Food microbiology. Tec and doc, Lavoisier. Paris,1997, 10-72.

G. Tadesse, E. Ephraim and M. Ashenafi, Assessment of the antimicrobial activity of lactic acid bacteria isolated from Borde and Shamita, traditional Ethiopian fermented beverages, on some foodborne pathogens and effect of growth medium on the inhibiting activity. Int.J. Food Safety N°5, 2004, 13-20.

NAID, 2014. National Agency of Investment Development: Interview with Djellaoui Abdelkader, Wali of Djelfa, Interview realized by ANDI with the Wali of Djelfa, March 2014.1-7.

BS 770-5. Methods for chemical analysis of cheese, determination of pH value Cheese, Dairy products, Processed Foods, Food products, Food testing, chemical analysis and testing, pH measurement, Calibration. ,1976, ISBN: 0 580 08747 6.

Z. Burdikova, Z. Svindrych, J. Pala, C.D. Hickey, M.G. Wilkinson, J. Panek, M.A.E. Auty, A. Periasamy and J.J. Sheehan, Measurement of pH micro-heterogeneity in natural cheese matrices by fluorescence lifetime imaging. Front Microbial., 2015, 6: 183.

M. Guetouache, B. Guessas, S. Medjekal and O. Toumatia. Technological and Biochemical characterization of Lactic Acid Bacteria isolated from Algerian Traditional Dairy Products. World Applied Sciences Journal, 2015, 33(2): 234-241.

L.Tudor, L.I. Ilie, E. Mitranescu, N. Ciocarlie and I. Logoe. The assessment of Microbiological quality of some traditional Romanian cheeses. lucrari stiinlifice medicina veterinara, 2009, XLII (2): 315-320.

F. Tasci, Microbiological and chemical properties of raw milk consumed in Burdur. Journal of Animal and Veterinary Advances, 2011, 10.5: 635-641.

G.M.S.C. Mata and M.C.D. Vanetti, Comparison of conventional and rapid methods for Salmonella detection in artisanal minas cheese. Journal of Food Research, 2012. 1(3): 178-183.

B. Nabaraj, J.S. Mallory and E.H.A. Healther, Diversity of Yeast and Mold Species from a Variety of Cheese Types. Faculty Publications in Food Science and Technology. Paper 140. Current Microbiology, 2015. pp:1-9.

GB 4789.15., National Food Safety Standard of P.R. China. Food Microbiological Examination: Enumeration of Yeasts and Molds; Issued Ministry of Health of P.R. China. 2010, ICS07. 100.30.C53.

C. Gusils, A.P. Chaia, G. Olivier and S. Gonzalez, Microtechnique for identification of lactic acid bacteria. Methods in molecular biology, Vol. 268: Public Health Microbiology: Methods and Protocols. Humana Press. Totowa, 2010. 453-458.

B.E. Terzaghi, and Sandine, W.E., “Improved medium for lactic enterococci and their bacteriophages”, Appl. Environ. Microbiol., 1975, 29, pp. 807-813.

P.R. Elliker, Anderson A.W. and Hannesson G, "An agar culture medium for lactic streptococci and lactobacilli". J. Dairy. Sci., 1956, 39, pp. 1611-1612.

J.V. Mayeux, Sandine, W.W.E. and Elliker P.R., "A selective medium for detecting Leuconostoc organisms in mixed strain starter cultures", J. Dairy. Sci., 1962. 45, pp. 655-656.

J. De Man, Rogosa, M. and Sharpe, M.E., “A medium for the cultivation of Lactobacilli”, J. Appl. Bacteriol., 1960. 23, pp. 130- 135.

J. L. Johnson, Phelips C. F., Cummins C. S., London J and Gasser F., "Taxonomy of Lactobacillus acidophilus group", Int. J. Syst. Bacteriol., 1980. 30, pp. 53-68.

G. Klein, "International Committee of Systematic Bacteriology. Subcommittee on the taxonomy of Bifidobacterium, Lactobacillus, and related organisms. Minutes of the meeting", Int. J. Syst. Evol. Microbiol., 2001. 51, pp. 259-261.

K. H. Schleifer, Kraus J., Dvorak C., Killper-Bâlz R., Collins M. D et Fisher W., "Transfer of Streptococcus lactis and related Streptococci to the genus Lactococcus gen, Nov", System. Appl. Microbiol., 1985. 6, pp. 183-195.

J. Samelis, Maurogenakis, F. et Metaxopoulos, J., “Characterization of lactic acid bacteria isolated from naturally fermented Greek dry salami”, Inter. J. Food. Microbiol., 1994. 23, pp. 179-196.

J. J. Devoyod, and Poullain, F., " The Properties of Leuconostocs: Their role in dairy technology”, Lait., 1988. 68, pp. 249-280.

F. Bissonnette, Labrie, S., Deveau, H., Lamoureux, M. and Moineau, S., “Characterization of mesophilic mixed starter cultures used for the manufacture of aged cheddar cheese”, J. Dairy Sci., 2000. Vol. 83, n° 4, pp.620-627.

T.D. Thomas, “Agar medium for differentiation of Streptococcus cremoris from the other bacteria”, N.Z.J. Dairy. Sci. Technol, 1973. 8, pp. 70-71.

J.B. Millière, Mathot, A.G., Schmitt, P. and Diviès C., “Phenotypic characterization of Leuconostoc species”, J. Appl. Bacteriol., 1989. 67, pp. 529-542.

P. Schmitt, Diviès, C. and Merlot, C., “Utilization of citrate by Leuconostoc mesenteroides subsp. cremoris in continuous culture”, Biotechnol. Lett.,1990.12, pp.127–130.

G.M. Kempler and Mc Kay, L.L., “Improved medium for detection of citrate-fermenting Streptococcus lactis subsp diacetylactis”, J. Appl. Environ. Microbiol. 1980.39, pp. 956- 927.

M. Keyvani, and Bolandi, Physicochemical and Organoleptic Properties of Lighvan Cheese Fortified with Protulaca Oleracea Seed Oil. Journal of Chemical Health Risks, 2015.5(1): 21-27.

Z. Mennane, M. Faid, M. Lagzouli, M. Ouhssine, M. Elyachioui, E. Berny, M. Ennouali and K. Khedid, Physicochemical, Microbial and Sensory Characterization of Moroccan Klila. Middle-East Journal of Scientific Research, 2007. 2(3-4): 93-97.

M.H. Rhiat, Labioui, A. Driouich, Z. Mennane and M. Ouhssine, Preparation of the starter trial production of cheeses (Jben) and (Klila) at laboratory scale. Food Sci. Qua. Manage., 2013. 13: 2225-0557.

F.P. Pacheco and A.B. Galindo. Microbial safety of raw milk cheeses traditionally made at a pH below 4.7 and with other hurdles limiting pathogen's growth. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. A. Méndez-Vilas (Ed), 2010. pp: 1205-1216.

A. Terzic-Vidojevic, K. Tonkovic, A.L. Pavunc, J. Beganovic, I. Strahinic, M. Kojic, K. Veljovic, N. Golic, B. Kos, N. Cadez, L. Gregurek, J. Suskovic J. Raspor and L.L. Topisirovic, Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses. LWT - Food Science and Technology, 2015. 63: 298-306.

EC (European Commission), Overview of Microbiological Criteria for Foodstuffs in Community Legislation in Force. 2001.

S.S. Kirdar, and O. Kursun, Microflora and pathogen bacteria in traditional Anatolian Canak (Pan) cheese. Journal of Animal and Veterinary Advances, 2011. 10(19): 2511-2515.

H. Hassanzadazar, and A. Ehsani, Phenotypic Characterization of Lactic Acid Bacteria Isolated from Traditional Koopeh Cheese. Global Veterinaria, 2013.10(2): 148-152.

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Published

2024-07-25

How to Cite

BENZEKRI , I., CHERIGUENE , A., CHOUGRANI , F., & DAHOU , A. E. (2024). Exploration of the biodiversity of the lactic flora of a traditional Algerian cheese (type: Jben) from the steppe zones of the Algerian center of Djelfa . International Journal of Advanced Natural Sciences and Engineering Researches, 8(6), 255–262. Retrieved from https://as-proceeding.com/index.php/ijanser/article/view/1953

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