Gene Expression of the MLH1 gene is regulated by non-coding RNA in patients with lung cancer
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Keywords:
Lung Cancer, Gene Expression, Non-Coding RNA Genes, MLH1 Gene, MMR System, Snorna-21 And FOXD2-AS1, Qrt-PCRAbstract
Lung cancer is a common cancer worldwide and can occur due to various causes, one of which is
the MMR mismatch repair system. This system repairs mismatched nucleotides in the DNA strand during
replication. MLH1, a key gene in the MMR system, is essential in repairing mutations in lung cancer
patients. Certain non-coding RNA genes, such as snoRNA and FOXD2-AS1, can regulate the expression of
this gene. These non-coding RNAs influence MLH1 gene expression in lung cancer patients. The
examination targeted at detecting the impact of miRNA and non-coding RNA on MLH1 gene expression
rates in lung cancer patients situated within Dohuk City. The study included 70 patients aged between 45 and
65 from September to October 2024, referred to AZADI Hospital. The sample was divided into two groups:
one included 55 lung cancer patients, another group included 15 individuals and was considered the control
group. A total of 5 ml of blood was drawn from each patient and segmented into two parts. The first part was
put in EDTA for DNA extraction, while the second part was placed in a Trizol tube for mRNA and non
coding RNA extraction. The study consisted of two stages: first, detecting the level of MLH1 gene
expression; second, assessing the level of non-coding RNA influencing gene activity. The results showed that
the MLH1 gene expression value was 0.38, while the gene expression values for non-coding RNAs were 4.4
for snoRNA and 4.2 for FOXD2-AS1.
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