Determination of antioxidant activity by chemical and electrochemical methods of extracts of Launaea resedifolia from the Algerian Sahara plant
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Keywords:Launaea resedifolia, Antioxidant activity, superoxide, cyclic voltammetry
The aim of this study is evaluating the antioxidant activity of the Launaea Resedifolia aerobic parts’ extracts, which grows in Bashar region (Algeria). The antioxidant properties of methanolic extract and the organic fractions of L. resedifolia have been estimated using the chemical and electrochemical methods. Where the chemical method is the superoxide scavenging assays, while the electrochemical method is the cyclic voltammetry assay. Further, both methods depend on the inhibition of superoxide anion radical. The results indicate that the extracts showed an inhibition of superoxide anion radical with an amount of I% ranging between (81.8181 ± 0.001) % and (66.6667 ± 5.2486) % in chemical method and IC50 between 3.2814 g/l and 1.7990 g/l in electrochemical method. Thus, the rate inhibition (I%) in the chemical method were found best in butanol fraction (BF) then chloroform fraction (CF) and ethyl acetate fraction (AF) then crud extract (CE). Both the effective scavenging concentration (IC50) in the electrochemical method were found best in ethyl acetate fraction (AF) then butanol fraction (BF), crud extract (CE) and chloroform fraction (CF).
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